Peripheral but not central leptin treatment increases numbers of circulating NK cells, granulocytes and specific monocyte subpopulations in non-endotoxaemic lean and obese LEW-rats
Philipp Haas, Rainer H Straub, Sammy Bedoui, Heike Nave
Peptides | ELSEVIER | Published : 2008
Leptin, a hormone mainly generated by adipocytes, acts centrally in the hypothalamus to regulate body weight and energy expenditure. However, there is strong evidence that leptin is also involved in cell-mediated immunity and cytokine crosstalk. In the present study the effects of diet-induced obesity and central and peripheral leptin treatment on leukocyte subsets and cytokine production was investigated. Leptin was injected either intravenously (i.v.) or intracerebroventricularly (i.c.v.) in male endotoxaemic or vehicle-treated healthy LEW-rats. Numbers of blood leukocyte subsets were analysed by FACS and cytokines (TNF-alpha and IL-6) by ELISA. Results showed that peripheral rather than c..View full abstract
The kind help with the intracerebroventricular cannulation of Prof. Dr. S. van Horsten (Section for Experimental Therapy, Friedrich-Alexander-University Erlangen-Nurnberg, Nurnberg, Germany) and the excellent technical assistance of S. Kuhlmann and 1. Dressendorfer are very much appreciated. This work was supported by a grant from the Eli Lilly International Foundation, Homburg, Germany.