Oct2 and Obf1 as facilitators of B:T cell collaboration during a humoral immune response
Lynn Corcoran, Dianne Emslie, Tobias Kratina, Wei Shi, Susanne Hirsch, Nadine Taubenheim, Stephane Chevrier
Frontiers in Immunology | FRONTIERS RESEARCH FOUNDATION | Published : 2014
The Oct2 protein, encoded by the Pou2f2 gene, was originally predicted to act as a DNA binding transcriptional activator of immunoglobulin (Ig) in B lineage cells. This prediction flowed from the earlier observation that an 8-bp sequence, the "octamer motif," was a highly conserved component of most Ig gene promoters and enhancers, and evidence from over-expression and reporter assays confirmed Oct2-mediated, octamer-dependent gene expression. Complexity was added to the story when Oct1, an independently encoded protein, ubiquitously expressed from the Pou2f1 gene, was characterized and found to bind to the octamer motif with almost identical specificity, and later, when the co-activator Obf..View full abstract
Awarded by NHMRC
We thank Drs. Laurie Glimcher for conditional Xbp1 mutant mice and Patrick Matthias for Obf1 mice, Gordon Smythe for bioinformatics assistance and Stephen Nutt for critical comments on the manuscript. Jennifer Vasiliadis and Louise Inglis provided expert animal care. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIIS and research grants from the NHMRC (#637306 and #575500).