Journal article

LIPOPOLYSACCHARIDE-GAMMA-INDUCED AND INTERFERON-GAMMA-INDUCED EXPRESSION OF HCK AND LYN TYROSINE KINASES IN MURINE BONE MARROW-DERIVED MACROPHAGES

I BOULET, S RALPH, E STANLEY, P LOCK, AR DUNN, SP GREEN, WA PHILLIPS

Oncogene | STOCKTON PRESS | Published : 1992

Abstract

We have examined the role of tyrosine phosphorylation during the course of macrophage activation. Initial experiments indicated that vanadate, a known phosphotyrosine phosphatase inhibitor, enhanced the phorbol 12-myristate 13-acetate (PMA)-triggered respiratory burst and potentiated the priming effects of bacterial lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma), suggesting that tyrosine phosphorylation may be important in these end cell functions. As src-related kinases have been implicated in the activation of cells of other haemopoietic lineages, we examined the relationship between the activity of two such kinases, hck and lyn, and priming of the respiratory burst. We found th..

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