Journal article

Prime-boost immunization generates a high frequency, high-avidity CD8( ) cytotoxic T lymphocyte population

MJ Estcourt, AJ Ramsay, A Brooks, SA Thomson, CJ Medveckzy, IA Ramshaw

International Immunology | OXFORD UNIV PRESS | Published : 2002


Development and expansion of high-avidity T cell populations may be important for the success of immunization strategies against HIV and other pathogens that have presented major problems for vaccine development. We have used tetrameric-MHC complexes ex vivo and lytic assays to show that 'prime-boost' immunization with DNA vaccines and recombinant poxvirus vectors generates high frequencies of cytotoxic T lymphocytes (CTL) that recognize target cells expressing very low levels of specific antigen. These cells persist for at least 6 months at levels representing approximately 10% of the CD8(+) T cell population. Using a novel in vivo assay, we also found that prime-boost immunized animals wer..

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University of Melbourne Researchers