Journal article

3D Electrospun scaffolds promote a cytotrophic phenotype of cultured primary astrocytes

Chew L Lau, Michelle Kovacevic, Tine S Tingleff, John S Forsythe, Holly S Cate, Daniel Merlo, Cecilia Cederfur, Francesca L Maclean, Clare L Parish, Malcolm K Horne, David R Nisbet, Philip M Beart



Astrocytes are a target for regenerative neurobiology because in brain injury their phenotype arbitrates brain integrity, neuronal death and subsequent repair and reconstruction. We explored the ability of 3D scaffolds to direct astrocytes into phenotypes with the potential to support neuronal survival. Poly-ε-caprolactone scaffolds were electrospun with random and aligned fibre orientations on which murine astrocytes were sub-cultured and analysed at 4 and 12 DIV. Astrocytes survived, proliferated and migrated into scaffolds adopting 3D morphologies, mimicking in vivo stellated phenotypes. Cells on random poly-ε-caprolactone scaffolds grew as circular colonies extending processes deep withi..

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Awarded by NHMRC

Funding Acknowledgements

The authors would like to thank Ms Alexandra Rodriguez and Kiara Bruggeman for critical reading of the manuscript. This research was supported by funding from an NHMRC project grant (APP1020332). DRN was supported by an Australian Research Council Australian Postdoctoral Fellowship and subsequently by an NHMRC Career Development Fellowship (APP1050684). MKH and PMB were supported NHMRC Research Fellowships, APP1020401 and APP1019833, respectively. CLP is a Senior Medical Research Fellow supported by the Viertel Charitable Foundation, Australia. Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged.