[3H] Adenosine uptake in brainstem membranes of CD-1 mice lacking the adenosine A2a receptor

Abstract

Previous studies in our laboratory have demonstrated a decrease in [ 3H]nitrobenzylthioinosine binding sites in the brainstem of adenosine A2a receptor knockout mice, particularly in the brain nuclei involved in central control of cardiovascular function [Brain Research 877 (2000) 160]. The present study aimed to correlate this decrease, shown using autoradiography, with a functional change using a previously described method of [3H]adenosine uptake in a membrane preparation from the brainstem of wildtype CD - 1 and homozygous mutant mice lacking the adenosine A2a receptor. A statistically significant decrease was shown in the mean V MAX value obtained from homozygous mutant preparations (4...