Anteroventral third ventricle (AV3V) lesion affects hypothalamic neuronal nitric oxide synthase (nNOS) expression following water deprivation

FA Aguila; GR Oliveira-Pelegrin; ST Yao; D Murphy; MJA Rocha

Journal article

Anteroventral third ventricle (AV3V) lesion affects hypothalamic neuronal nitric oxide synthase (nNOS) expression following water deprivation

FA Aguila, GR Oliveira-Pelegrin, ST Yao, D Murphy, MJA Rocha

Brain Research Bulletin | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2011

DOI: 10.1016/j.brainresbull.2011.07.020

Abstract

Neuronal nitric oxide synthase (nNOS) has been reported to be up-regulated in the hypothalamic supraoptic nucleus (SON) during dehydration which in turn could increase nitric oxide (NO) production and consequently affect arginine vasopressin (AVP) secretion. The anteroventral third ventricle (AV3V) region has strong afferent connections with the SON. Herein we describe our analysis of the effects of an AV3V lesion on AVP secretion, and c-fos and nNOS expression in the SON following dehydration. Male Wistar rats had their AV3V region electrolytically lesioned or were sham operated. After 21 days they were submitted to dehydration or left as controls (euhydrated). Two days later, one group was..

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University of Melbourne Researchers

Song Yao Author

Grants

Funding Acknowledgements

The authors thank Nadir M. Fernandes, Milene M. Lopes for technical assistance. We also thank Drs. Jose Antunes-Rodrigues and Lucila L.K. Elias, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, for providing the infrastructure for the RIA analyses. Financial support from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) is gratefully acknowledged.