Journal article

Coronin 3 involvement in F-actin-dependent processes at the cell cortex

Andre Rosentreter, Andreas Hofmann, Charles-Peter Xavier, Maria Stumpf, Angelika A Noegel, Christoph S Clemen

Experimental Cell Research | ELSEVIER INC | Published : 2007

Abstract

The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins and RNAi silencing we show that coronin 3 has roles in wound healing, protrusion formation, cell proliferation, cytokinesis, endocytosis, axonal growth, and secretion. During formation of cell protrusions actin accumulation precedes the focal enrichment of coronin 3 suggesting a role for coronin 3 in events that follow the initial F-actin assembly. Moreover, we show that coronin..

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University of Melbourne Researchers