A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum
Matthew WA Dixon, Christopher L Peatey, Donald L Gardiner, Katharine R Trenholme
Molecular and Biochemical Parasitology | ELSEVIER SCIENCE BV | Published : 2009
Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent.
MD was supported by an Australia and New Zealand Trustees PHD scholarship. CP is supported by an Australian Postgraduate Award and a Dr Diana Cavaye Scholarship. DG is supported by an Australian National Health and Medical Research Council Career Development Award. KT and DG would like to acknowledge a generous donation from Mark Nicholson, Alice Hill and the Tudor Foundation.