Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes
Francis HW Shand, Satoshi Ueha, Mikiya Otsuji, Suang Suang Koid, Shigeyuki Shichino, Tatsuya Tsukui, Mizuha Kosugi-Kanaya, Jun Abe, Michio Tomura, James Ziogas, Kouji Matsushima
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2014
Myeloid cells such as monocytes and monocyte-derived macrophages promote tumor progression. Recent reports suggest that extramedullary hematopoiesis sustains a sizable reservoir of tumor-infiltrating monocytes in the spleen. However, the influence of the spleen on tumor development and the extent to which spleen monocytes populate the tumor relative to bone marrow (BM) monocytes remain controversial. Here, we used mice expressing the photoconvertible protein Kikume Green-Red to track the redistribution of monocytes from the BM and spleen, and mice expressing fluorescent ubiquitination-based cell-cycle indicator proteins to monitor active hematopoiesis in these tissues. In mice bearing late-s..View full abstract
Awarded by Japanese Ministry of Education, Culture, Sports, Science and Technology
Awarded by Grants-in-Aid for Scientific Research
We thank A. Miyawaki and the RIKEN BioResource Center for providing FucciG<INF>1</INF> and FucciS/G<INF>2</INF>/M mice; C. Kasahara and A. Hata for animal care; S. Aoki and S. Fujita for expert technical assistance; and I. Bertoncello for helpful discussions. This work was supported by the Japan Science and Technology Agency CREST program; Grants-in-Aid for Scientific Research (C) 25460491 (to S.U.) and (B) 25293113 (to K.M.) from the Japanese Ministry of Education, Culture, Sports, Science and Technology; an Australian Government Prime Minister's Australia Asia Endeavour Award (to F.H.W.S.); and an E & V Puzey Foundation Scholarship (to F.H.W.S.).