Journal article
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes
FHW Shand, S Ueha, M Otsuji, SS Koid, S Shichino, T Tsukui, M Kosugi-Kanaya, J Abe, M Tomura, J Ziogas, K Matsushima
Proceedings of the National Academy of Sciences of the United States of America | Published : 2014
Abstract
Myeloid cells such as monocytes and monocyte-derived macrophages promote tumor progression. Recent reports suggest that extramedullary hematopoiesis sustains a sizable reservoir of tumor-infiltrating monocytes in the spleen. However, the influence of the spleen on tumor development and the extent to which spleen monocytes populate the tumor relative to bone marrow (BM) monocytes remain controversial. Here, we used mice expressing the photoconvertible protein Kikume Green-Red to track the redistribution of monocytes from the BM and spleen, and mice expressing fluorescent ubiquitination-based cell-cycle indicator proteins to monitor active hematopoiesis in these tissues. In mice bearing late-s..
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Awarded by Japan Society for the Promotion of Science
Funding Acknowledgements
We thank A. Miyawaki and the RIKEN BioResource Center for providing FucciG<INF>1</INF> and FucciS/G<INF>2</INF>/M mice; C. Kasahara and A. Hata for animal care; S. Aoki and S. Fujita for expert technical assistance; and I. Bertoncello for helpful discussions. This work was supported by the Japan Science and Technology Agency CREST program; Grants-in-Aid for Scientific Research (C) 25460491 (to S.U.) and (B) 25293113 (to K.M.) from the Japanese Ministry of Education, Culture, Sports, Science and Technology; an Australian Government Prime Minister's Australia Asia Endeavour Award (to F.H.W.S.); and an E & V Puzey Foundation Scholarship (to F.H.W.S.).