Journal article

Determining Lineage Pathways from Cellular Barcoding Experiments

Leila Perie, Philip D Hodgkin, Shalin H Naik, Ton N Schumacher, Rob J de Boer, Ken R Duffy

Cell Reports | CELL PRESS | Published : 2014

Abstract

Cellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in vivo cell fate that have been instrumental in several significant recent discoveries. Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the resulting data for evaluation of potential lineage pathways requires a new quantitative framework complete with appropriate statistical tests. Here, we develop such a framework, illustrating its utility by analyzing data from barcoded multipotent cells of the blood system. This application demonstrates that the data require additional paths beyond those found in the classical model, which leads us ..

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Grants

Awarded by Human Frontier Science Program


Awarded by Nederlandse Organisatie voor Wetenschappelijk Onderzoek TOP GO


Awarded by Science Foundation Ireland


Awarded by National Science Foundation from the Kavli Institute for Theoretical Physics


Funding Acknowledgements

We thank T. Krohn for helpful feedback on the manuscript and L. Riboli-Sasco for useful discussions. L.P. was supported by a Marie Curie Intra European FP7 fellowship and a Bettencourt-Schueller foundation award. The work of P.H., S.N., T.S., and K.D. was supported by Human Frontier Science Program grant RGP0060/2012. R.D.B. and T.S. were supported by a Nederlandse Organisatie voor Wetenschappelijk Onderzoek TOP GO grant (TOPGO.L.10.042). K.D. was also supported by Science Foundation Ireland grant 12 IP 1263. This research was supported in part by the National Science Foundation under grant NSF PHY11-25915 from the Kavli Institute for Theoretical Physics.