Journal article

High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi

Kathryn E Holt, Julian Parkhill, Camila J Mazzoni, Philippe Roumagnac, Francois-Xavier Weill, Ian Goodhead, Richard Rance, Stephen Baker, Duncan J Maskell, John Wain, Christiane Dolecek, Mark Achtman, Gordon Dougan

Nature genetics | NATURE PUBLISHING GROUP | Published : 2008


Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination between isolates. Rather, evolution in the Typhi population seems to be characterized by ongoing loss of gene function, consistent with a small effective population size. The lack of evidence for antigeni..

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University of Melbourne Researchers