Analysis of the Vaccine Potential of Plasmid DNA Encoding Nine Mycolactone Polyketide Synthase Domains in Mycobacterium ulcerans Infected Mice
Virginie Roupie, Sacha J Pidot, Tobba Einarsdottir, Christophe Van den Poel, Fabienne Jurion, Timothy P Stinear, Kris Huygen
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2014
There is no effective vaccine against Buruli ulcer. In experimental footpad infection of C57BL/6 mice with M. ulcerans, a prime-boost vaccination protocol using plasmid DNA encoding mycolyltransferase Ag85A of M. ulcerans and a homologous protein boost has shown significant, albeit transient protection, comparable to the one induced by M. bovis BCG. The mycolactone toxin is an obvious candidate for a vaccine, but by virtue of its chemical structure, this toxin is not immunogenic in itself. However, antibodies against some of the polyketide synthase domains involved in mycolactone synthesis, were found in Buruli ulcer patients and healthy controls from the same endemic region, suggesting that..View full abstract
Awarded by BuruliVac, Identification and development of vaccine candidates for Buruli Ulcer Disease
This work was funded by BuruliVac, Identification and development of vaccine candidates for Buruli Ulcer Disease (Grant agreement FP7 241500, www.burulivac.eu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.