Journal article

CATION FLUXES AND VOLUME REGULATION BY HUMAN-LYMPHOCYTES

AH BUI, JS WILEY

Journal of Cellular Physiology | WILEY | Published : 1981

Abstract

The ionic basis of volume regulation by human peripheral blood lymphocytes in hypotonic Tyrode's medium has been studied. The intracellular water space of lymphocytes increased to a maximum after 1 min in 0.68 X isotonic Tyrode's but returned to the isotonic value by 20 min at 37 degrees C. During this phase of volume regulation (1-20 min) both 42K+ efflux and 42K+ influx were stimulated severalfold, but the increase in 42K+ efflux exceeded the influx, resulting in a net loss of 20% of the lymphocyte K+. The increase in 42K+ efflux during the phase of cell shrinkage was unaffected by ouabain or by quinidine. Hypotonicity increased both the ouabain-sensitive (active) and ouabain-insensitive c..

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