Journal article

Differential sensitivity of culture and the polymerase chain reaction for detection of feline herpesvirus 1 in vaccinated and unvaccinated cats

JE Sykes, GF Browning, G Anderson, VP Studdert, HV Smith

Archives of Virology | SPRINGER-VERLAG WIEN | Published : 1997


The diagnostic sensitivities of the polymerase chain reaction (PCR) and culture were compared and correlated with clinical signs in 5 vaccinated cats and 3 unvaccinated cats that were experimentally infected with feline herpesvirus 1. Conjunctival swabs were taken each day from 0 to 14 days and on 21, 28 and 30 days after challenge. PCR (49.3%) was significantly more sensitive than culture (30.1%) as assessed by an adjusted McNemar's test to account for non-independence of results between days within each cat (P = 0.02). PCR was considerably more sensitive (34.1%) than culture (8.2%) in vaccinated cats (P = 0.001), whereas there was no significant difference in sensitivities in the unvaccina..

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