Journal article

The Circadian Clock in Murine Chondrocytes Regulates Genes Controlling Key Aspects of Cartilage Homeostasis

Nicole Gossan, Leo Zeef, James Hensman, Alun Hughes, John F Bateman, Lynn Rowley, Christopher B Little, Hugh D Piggins, Magnus Rattray, Raymond P Boot-Handford, Qing-Jun Meng

Arthritis and Rheumatism | WILEY | Published : 2013

Abstract

OBJECTIVE: To characterize the circadian clock in murine cartilage tissue and identify tissue-specific clock target genes, and to investigate whether the circadian clock changes during aging or during cartilage degeneration using an experimental mouse model of osteoarthritis (OA). METHODS: Cartilage explants were obtained from aged and young adult mice after transduction with the circadian clock fusion protein reporter PER2::luc, and real-time bioluminescence recordings were used to characterize the properties of the clock. Time-series microarrays were performed on mouse cartilage tissue to identify genes expressed in a circadian manner. Rhythmic genes were confirmed by quantitative reverse ..

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Grants

Awarded by Wellcome Trust, UK


Awarded by National Health and Medical Research Council of Australia


Awarded by Biotechnology and Biological Sciences Research Council, UK


Awarded by Medical Research Council, UK


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Medical Research Council


Funding Acknowledgements

Supported by the Wellcome Trust, UK (core funding grant 088785/Z/09/Z to the University of Manchester Wellcome Trust Centre for Cell-Matrix Research), the National Health and Medical Research Council of Australia (grant 607399), the Victorian Government, Australia (Operational Infrastructure Support Program funding), the Biotechnology and Biological Sciences Research Council, UK (grant BB/H018123/2 to Dr. Rattray), the University of Manchester (Promoting Interface Networking Award to Drs. Boot-Handford and Meng), and the Medical Research Council, UK (Career Development Award G0900414 to Dr. Meng).