Journal article

Sorting of Growth Plate Chondrocytes Allows the Isolation and Characterization of Cells of a Defined Differentiation Status

Daniele Belluoccio, Julia Etich, Sabrina Rosenbaum, Christian Frie, Ivan Grskovic, Jacek Stermann, Harald Ehlen, Simon Vogel, Frank Zaucke, Klaus von der Mark, John F Bateman, Bent Brachvogel

JOURNAL OF BONE AND MINERAL RESEARCH | WILEY | Published : 2010

Abstract

Axial growth of long bones occurs through a coordinated process of growth plate chondrocyte proliferation and differentiation. This maturation of chondrocytes is reflected in a zonal change in gene expression and cell morphology from resting to proliferative, prehypertrophic, and hypertrophic chondrocytes of the growth plate followed by ossification. A major experimental limitation in understanding growth plate biology and pathophysiology is the lack of a robust technique to isolate cells from the different zones, particularly from small animals. Here, we report on a new strategy for separating distinct chondrocyte populations from mouse growth plates. By transcriptome profiling of microdiss..

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