Sorting of Growth Plate Chondrocytes Allows the Isolation and Characterization of Cells of a Defined Differentiation Status
Daniele Belluoccio, Julia Etich, Sabrina Rosenbaum, Christian Frie, Ivan Grskovic, Jacek Stermann, Harald Ehlen, Simon Vogel, Frank Zaucke, Klaus von der Mark, John F Bateman, Bent Brachvogel
JOURNAL OF BONE AND MINERAL RESEARCH | WILEY | Published : 2010
Axial growth of long bones occurs through a coordinated process of growth plate chondrocyte proliferation and differentiation. This maturation of chondrocytes is reflected in a zonal change in gene expression and cell morphology from resting to proliferative, prehypertrophic, and hypertrophic chondrocytes of the growth plate followed by ossification. A major experimental limitation in understanding growth plate biology and pathophysiology is the lack of a robust technique to isolate cells from the different zones, particularly from small animals. Here, we report on a new strategy for separating distinct chondrocyte populations from mouse growth plates. By transcriptome profiling of microdiss..View full abstract
Awarded by Deutsche Forschungsgemeinschaft
We gratefully acknowledge the scientific advice of Ernst Poeschl (University of East Anglia, Norwich, United Kingdom) and would like to thank Reinhard Fassler (Max Planck Institute of Biochemistry, Munich, Germany) for providing the Col9a1-deficient mice. This project was supported by grants from the Deutsche Forschungsgemeinschaft (BR2304/4-1 and SFB829-B6).