gamma delta T cells and CD14( ) Monocytes Are Predominant Cellular Sources of Cytokines and Chemokines Associated With Severe Malaria
Danielle I Stanisic, Julia Cutts, Emily Eriksson, Freya JI Fowkes, Anna Rosanas-Urgell, Peter Siba, Moses Laman, Timothy ME Davis, Laurens Manning, Ivo Mueller, Louis Schofield
The Journal of Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2014
BACKGROUND: Severe malaria (SM) is associated with high levels of cytokines such as tumor necrosis factor (TNF), interleukin 1 (IL-1), and interleukin 6 (IL-6). The role of chemokines is less clear, as is their cellular source. METHODS: In a case-control study of children with SM (n = 200), uncomplicated malaria (UM) (n = 153) and healthy community controls (HC) (n = 162) in Papua, New Guinea, we measured cytokine/chemokine production by peripheral blood mononuclear cells (PBMCs) stimulated with live Plasmodium falciparum parasitized red blood cells (pRBC). Cellular sources were determined. Associations between immunological endpoints and clinical/parasitological variables were tested. RESUL..View full abstract
Awarded by NHMRC Practitioner Fellowship
This work was supported by a National Health and Medical Research Council (NHMRC) training award (F. J. I. F.), NHMRC scholarship (L. M.), NHMRC Practitioner Fellowship (T. M. E. D.), Project Grants 516735, 513782 and Program Grant 637406. L. S. was supported by an International Research Scholarship of the Howard Hughes Medical Institute. L. M. was supported by a Basser Scholarship from the Royal Australasian College of Physicians. M. L. was supported by Fogarty Foundation Scholarship. The authors acknowledge support from the Malaria Genomic Epidemiology Network (MalariaGEN).