CDC25B Overexpression Stabilises Centrin 2 and Promotes the Formation of Excess Centriolar Foci
Rose Boutros, Odile Mondesert, Corinne Lorenzo, Puji Astuti, Grant McArthur, Megan Chircop, Bernard Ducommun, Brian Gabrielli
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2013
CDK-cyclin complexes regulate centriole duplication and microtubule nucleation at specific cell cycle stages, although their exact roles in these processes remain unclear. As the activities of CDK-cyclins are themselves positively regulated by CDC25 phosphatases, we investigated the role of centrosomal CDC25B during interphase. We report that overexpression of CDC25B, as is commonly found in human cancer, results in a significant increase in centrin 2 at the centrosomes of interphase cells. Conversely, CDC25B depletion causes a loss of centrin 2 from the centrosome, which can be rescued by treatment with the proteasome inhibitor MG132. CDC25B overexpression also promotes the formation of exc..View full abstract
RB was supported by a C.J. Martin post-doctoral training fellowship and a Project Grant from the National Health and Medical Research Council (NHMRC) Australia. Work in the Gabrielli lab is supported by the NHMRC Australia. Work in the Ducommun lab was supported by C.N.R.S, l'Universite Paul Sabatier and la Ligue Nationale Contre le Cancer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.