Sustained function of genetically modified porcine lungs in an ex vivo model of pulmonary xenotransplantation
Glen P Westall, Browyn J Levvey, Evelyn Salvaris, Julian Gooi, Sylvana Marasco, Frank Rosenfeldt, Chris Egan, Robin McEgan, Mark Mennen, Prue Russell, Simon C Robson, Mark B Nottle, Karen M Dwyer, Greg I Snell, Peter J Cowan
JOURNAL OF HEART AND LUNG TRANSPLANTATION | ELSEVIER SCIENCE INC | Published : 2013
BACKGROUND: Xenotransplantation could provide a solution to the donor shortage that is currently the major barrier to solid-organ transplantation. The ability to breed pigs with multiple genetic modifications provides a unique opportunity to explore the immunologic challenges of pulmonary xenotransplantation. METHODS: Explanted lungs from wild-type and 3 groups of genetically modified pigs were studied: (i) α1,3-galactosyltransferase gene knockout (GTKO); (ii) GTKO pigs expressing the human complementary regulatory proteins CD55 and CD59 (GTKO/CD55-59); and (iii) GTKO pigs expressing both CD55-59 and CD39 (GTKO/CD55-59/CD39). The physiologic, immunologic and histologic properties of porcine ..View full abstract
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
This study was supported by a Norman E. Shumway International Society for Heart and Lung Transplantation (ISHLT) Career Development Award (to G.W.) and by the Margaret Pratt Foundation.