Development of Retroviral Vectors for Tissue-Restricted Expression in Chicken Embryonic Gonads
Luke S Lambeth, Thomas Ohnesorg, David M Cummins, Andrew H Sinclair, Craig A Smith
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014
The chicken embryo has long been a useful model organism for studying development, including sex determination and gonadal differentiation. However, manipulating gene expression specifically in the embryonic avian gonad has been difficult. The viral vector RCASBP can be readily used for embryo-wide transgene expression; however global mis-expression using this method can cause deleterious off-target effects and embryo-lethality. In an attempt to develop vectors for the over-expression of sequences in chicken embryonic urogenital tissues, the viral vector RCANBP was engineered to contain predicted promoter sequences of gonadal-expressed genes. Several promoters were analysed and it was found ..View full abstract
Awarded by National Health and Medical Research Council
This work was supported by an Australian Research Council (ARC) Future Fellowship awarded to CAS and The National Health and Medical Research Council (Project Grant # 1031214) awarded to TO. No additional external funding was received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.