Journal article

A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere

AWI Lo, JM Craig, R Saffery, P Kalitsis, DV Irvine, E Earle, DJ Magliano, KHA Choo

EMBO JOURNAL | WILEY | Published : 2001


Centromere protein A (CENP-A) is an essential centromere-specific histone H3 homologue. Using combined chromatin immunoprecipitation and DNA array analysis, we have defined a 330 kb CENP-A binding domain of a 10q25.3 neocentromere found on the human marker chromosome mardel(10). This domain is situated adjacent to the 80 kb region identified previously as the neocentromere site through lower-resolution immunofluorescence/FISH analysis of metaphase chromosomes. The 330 kb CENP-A binding domain shows a depletion of histone H3, providing evidence for the replacement of histone H3 by CENP-A within centromere-specific nucleosomes. The DNA within this domain has a high AT-content comparable to tha..

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