MRI-based glomerular morphology and pathology in whole human kidneys
Scott C Beeman, Luise A Cullen-McEwen, Victor G Puelles, Min Zhang, Teresa Wu, Edwin J Baldelomar, John Dowling, Jennifer R Charlton, Michael S Forbes, Amanda Ng, Qi-zhu Wu, James A Armitage, Gary F Egan, John F Bertram, Kevin M Bennett
American Journal of Physiology. Renal physiology | AMER PHYSIOLOGICAL SOC | Published : 2014
Nephron number (N(glom)) and size (V(glom)) are correlated with risk for chronic cardiovascular and kidney disease and may be predictive of renal allograft viability. Unfortunately, there are no techniques to assess N(glom) and V(glom) in intact kidneys. This work demonstrates the use of cationized ferritin (CF) as a magnetic resonance imaging (MRI) contrast agent to measure N(glom) and V(glom) in viable human kidneys donated to science. The kidneys were obtained from patients with varying levels of cardiovascular and renal disease. CF was intravenously injected into three viable human kidneys. A fourth control kidney was perfused with saline. After fixation, immunofluorescence and electron ..View full abstract
Awarded by NIH
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
G. F. Egan is a National Health and Medical Research Council Principal Research Fellow. This work was funded by a grant to K. M. Bennett from the National Institutes of Health (NIH) Diabetic Complications Consortium and by NIH Grant DK-091722. V. G. Puelles received a Monash Research Graduate School Scholarship and a Faculty of Medicine International Postgraduate Scholarship to support his PhD candidature.