Journal article

Comparison of an assumed versus measured leucocyte count in parasite density calculations in Papua New Guinean children with uncomplicated malaria

Moses Laman, Brioni R Moore, John Benjamin, Nixon Padapu, Nandao Tarongka, Peter Siba, Inoni Betuela, Ivo Mueller, Leanne J Robinson, Timothy ME Davis

Malaria Journal | BMC | Published : 2014

Abstract

BACKGROUND: The accuracy of the World Health Organization method of estimating malaria parasite density from thick blood smears by assuming a white blood cell (WBC) count of 8,000/μL has been questioned in several studies. Since epidemiological investigations, anti-malarial efficacy trials and routine laboratory reporting in Papua New Guinea (PNG) have all relied on this approach, its validity was assessed as part of a trial of artemisinin-based combination therapy, which included blood smear microscopy and automated measurement of leucocyte densities on Days 0, 3 and 7. RESULTS: 168 children with uncomplicated malaria (median (inter-quartile range) age 44 (39-47) months) were enrolled, 80.3..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by NHMRC


Funding Acknowledgements

We thank the patients and their families for their participation. We also gratefully acknowledge the assistance of field staff at Mugil and Alexishafen Health Centres, and the Microscopy Unit at the Vector Borne Diseases Unit, PNG Institute of Medical Research. This study was funded by a National Health and Medical Research Council (NHMRC) of Australia project grant (#634343). ML was supported by an Australian Award PhD Scholarship, BRM by an NHMRC Early Career Fellowship (#1036951), IM by a NHMRC Senior Research Fellowship (#1043345), LJR by an NHMRC Early Career Fellowship (#1016443) and TMED by an NHMRC Practitioner Fellowship (#572561).