Journal article

Strategies for Detection of Plasmodium species Gametocytes

Rahel Wampfler, Felistas Mwingira, Sarah Javati, Leanne Robinson, Inoni Betuela, Peter Siba, Hans-Peter Beck, Ivo Mueller, Ingrid Felger

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2013

Abstract

Carriage and density of gametocytes, the transmission stages of malaria parasites, are determined for predicting the infectiousness of humans to mosquitoes. This measure is used for evaluating interventions that aim at reducing malaria transmission. Gametocytes need to be detected by amplification of stage-specific transcripts, which requires RNA-preserving blood sampling. For simultaneous, highly sensitive quantification of both, blood stages and gametocytes, we have compared and optimized different strategies for field and laboratory procedures in a cross sectional survey in 315 5-9 yr old children from Papua New Guinea. qRT-PCR was performed for gametocyte markers pfs25 and pvs25, Plasmod..

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Grants

Awarded by Swiss National Science Foundation


Awarded by International Centers of Excellence in Malaria Research


Awarded by Brazilian Swiss Joint Research Programme


Awarded by Australian National Health and Medical Research Council


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by the Swiss National Science Foundation (grant no. 310030_134889), the International Centers of Excellence in Malaria Research (grant no. U19 AI089686-03) and the Brazilian Swiss Joint Research Programme (grant no. BJRP 0112-07). L. Robinson was supported by an Australian National Health and Medical Research Council Early Career Fellowship #1016443. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.