Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay
Carmen Fernandez-Becerra, Sergi Sanz, Marina Brucet, Danielle I Stanisic, Fabiana P Alves, Erney P Camargo, Pedro L Alonso, Ivo Mueller, Hernando A del Portillo
Malaria Journal | BMC | Published : 2010
BACKGROUND: Progress towards the development of a malaria vaccine against Plasmodium vivax, the most widely distributed human malaria parasite, will require a better understanding of the immune responses that confer clinical protection to patients in regions where malaria is endemic. METHODS: Glutathione S-transferase (GST) and GST-fusion proteins representing the N- terminus of the merozoite surface protein 1 of P. vivax, PvMSP1-N, and the C-terminus, PvMSP1-C, were covalently coupled to BioPlex carboxylated beads. Recombinant proteins and coupled beads were used, respectively, in ELISA and Bioplex assays using immune sera of P. vivax patients from Brazil and PNG to determine IgG and subcla..View full abstract
We are particularly grateful to all the patients and healthy volunteers that participated of this study. Studies in PNG and Brazil received ethical approval from local Institutional Reviewing Boards. Work in the laboratory of HAP is funded by the Fundacion Privada CELLEX (Catalonia, Spain).