Silencing and Nuclear Repositioning of the lambda 5 Gene Locus at the Pre-B Cell Stage Requires Aiolos and OBF-1
Alexander Karnowski, Chun Cao, Gabriele Matthias, Sebastian Carotta, Lynn M Corcoran, Inga-Lill Martensson, Jane A Skok, Patrick Matthias
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2008
The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expressio..View full abstract
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
This work was funded by the Novartis Research Foundation (to PM), the BBSRC, UK (to ILM) and The Wellcome Trust Fund (to JAS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.