Journal article

Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins

AM Verhagen, PG Ekert, M Pakusch, J Silke, LM Connolly, GE Reid, RL Moritz, RJ Simpson, DL Vaux

Cell | CELL PRESS | Published : 2000

Abstract

To identify proteins that bind mammalian IAP homolog A (MIHA, also known as XIAP), we used coimmuno-precipitation and 2D immobilized pH gradient/SDS PAGE, followed by electrospray ionization tandem mass spectrometry. DIABLO (direct IAP binding protein with low pI) is a novel protein that can bind MIHA and can also interact with MIHB and MIHC and the baculoviral IAP, OpIAP. The N-terminally processed, IAP-interacting form of DIABLO is concentrated in membrane fractions in healthy cells but released into the MIHA-containing cytosolic fractions upon UV irradiation. As transfection of cells with DIABLO was able to counter the protection afforded by MIHA against UV irradiation, DIABLO may promote..

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