Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

Abstract

Objective: To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome. Design: Experimental study. Setting: Research laboratory. Animal(s): Four- to five-week-old F1 (C57BL/6 × CBA/Ca) female mice. Intervention(s): Analysis by time-of-flight mass spectrometry. Main Outcome Measure(s): Protein profiles of denuded mouse metaphase II (MII) oocytes. Result(s): Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e., equilibration, dehydration with or without seeding) exhibited significantly altered protein profiles in comparison to controls (i.e., 37°C for 20 minutes and room temperature for 20 min). In total, 11 protein-biomarker..