Journal article

Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

Mandy G Katz-Jaffe, Mark G Larman, Courtney B Sheehan, David K Gardner

Fertility and Sterility | ELSEVIER SCIENCE INC | Published : 2008

Abstract

OBJECTIVE: To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome. DESIGN: Experimental study. SETTING: Research laboratory. ANIMAL(S): Four- to five-week-old F1 (C57BL/6 x CBA/Ca) female mice. INTERVENTION(S): Analysis by time-of-flight mass spectrometry. MAIN OUTCOME MEASURE(S): Protein profiles of denuded mouse metaphase II (MII) oocytes. RESULT(S): Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e., equilibration, dehydration with or without seeding) exhibited significantly altered protein profiles in comparison to controls (i.e., 37 degrees C for 20 minutes and room temperature for 20 min). In total, 11 protein-b..

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University of Melbourne Researchers