Deletion of the SOCS box of suppressor of cytokine signaling 3 (SOCS3) in embryonic stem cells reveals SOCS box-dependent regulation of JAK but not STAT phosphorylation
Kristy Boyle, Jian-Guo Zhang, Sandra E Nicholson, Evelyn Trounson, Jeffery J Babon, Edward J McManus, Nicos A Nicola, Lorraine Robb
CELLULAR SIGNALLING | ELSEVIER SCIENCE INC | Published : 2009
The mechanism by which Suppressor of Cytokine Signaling-3 (SOCS3) negatively regulates cytokine signaling has been widely investigated using over-expression studies in cell lines and is thought to involve interactions with both the gp130 receptor and JAK1. Here, we compare the endogenous JAK/STAT signaling pathway downstream of Leukemia Inhibitory Factor (LIF) signaling in wild type (WT) Embryonic Stem (ES) cells and in ES cells lacking either the entire Socs3 gene or bearing a truncated form of SOCS3 (SOCS3DeltaSB) lacking the C-terminal SOCS box motif (SOCS3(DeltaSB/DeltaSB)). In SOCS3(DeltaSB/DeltaSB) cells phosphorylated JAK1 accumulated at much higher levels than in WT cells or even cel..View full abstract
Awarded by National Institute of Health
Awarded by National Health and Medical Research Council of Australia
Awarded by NATIONAL CANCER INSTITUTE
This work was supported by grants from the National Institute of Health (CA-22556) and the National Health and Medical Research Council of Australia (Program Grant 461219).