Journal article
γH2AX as a molecular marker of aging and disease
LJ Mah, A El-Osta, TC Karagiannis
Epigenetics | TAYLOR & FRANCIS INC | Published : 2010
Abstract
Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed. © 2010 Landes Bioscience.
Grants
Awarded by University of Melbourne
Funding Acknowledgements
The support of the Australian Institute of Nuclear Science and Engineering is acknowledged. T. C. K. was the recipient of AINSE awards. Molecular Radiation Biology is supported by the National Health and Medical Research Council of Australia (566559). L. M. is supported by Melbourne Research (University of Melbourne) and Biomedical Imaging CRC supplementary scholarships.