Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach
Hamish EG McWilliam, Patrick Driguez, David Piedrafita, Donald P McManus, Els NT Meeusen
Parasites and Vectors | BMC | Published : 2014
BACKGROUND: Novel vaccine candidates against Schistosoma japonicum are required, and antigens present in the vulnerable larval developmental stage are attractive targets. Post-genomic technologies are now available which can contribute to such antigen discovery. METHODS: A schistosome-specific protein microarray was probed using the local antibody response against migrating larvae. Antigens were assessed for their novelty and predicted larval expression and host-exposed features. One antigen was further characterised and its sequence and structure were analysed in silico. Real-time polymerase chain reaction was used to analyse transcript expression throughout development, and immunoblotting ..View full abstract
Awarded by NHMRC
We wish to acknowledge Mary Duke for her S. japonicum infection and animal handling expertise, and financial support from the National Health and Medical Research Council of Australia and the Australian Research Council Centre of Excellence in Structural & Functional Microbial Genomics. HM was supported by a NHMRC postgraduate scholarship (number 607203). DPM is a NHMRC Senior Principal Research Fellow.