Journal article

In vitro function and phagocytosis of galactosylated platelet concentrates after long-term refrigeration

Aleksandar M Babic, Emma C Josefsson, Wolfgang Bergmeier, Denisa D Wagner, Richard M Kaufman, Leslie E Silberstein, Thomas P Stossel, John H Hartwig, Karin M Hoffmeister

Transfusion | WILEY | Published : 2007

Abstract

BACKGROUND: Short-term refrigeration of platelets (PLTs) in the absence of plasma results in their rapid clearance after transfusion. Blocking beta-N-acetylglucosamine (beta-GlcNAc) residues of glycoprotein Ibalpha (GPIbalpha) with galactose prevents binding of refrigerated human and mouse PLTs to macrophages and prolongs the circulation times of refrigerated mouse PLTs. PLT-associated galactosyltransferase efficiently galactosylates chilled PLTs in the presence of its substrate UDP-galactose is added to PLT-rich plasma. STUDY DESIGN AND METHODS: To characterize the hemostatic function of refrigerated and galactosylated human PLTs processed in the blood bank, PLT aggregation was studied in v..

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