Investigation of trefoil factor expression in saliva and oral mucosal tissues of patients with oral squamous cell carcinoma
Ponlatham Chaiyarit, Akasith Utrawichian, Chanvit Leelayuwat, Patrawut Vatanasapt, Nattharee Chanchareonsook, Mie Hessellund Samson, Andrew S Giraud
CLINICAL ORAL INVESTIGATIONS | SPRINGER HEIDELBERG | Published : 2012
OBJECTIVES: The aims of our study were to determine levels of trefoil factor (TFF) peptides in saliva and oral mucosal tissues from patients with oral squamous cell carcinoma (OSCC), and to evaluate whether individual members of TFFs (TFF1, TFF2, and TFF3) might act as biomarkers of disease. MATERIALS AND METHODS: Saliva samples were from 23 healthy subjects and 23 OSCC patients. Tissue samples were collected from 32 normal oral mucosa (NOM) and 32 OSCC biopsy specimens. ELISA and immunohistochemical methods were used to evaluate the expression of TFF1, TFF2, and TFF3 in saliva and oral mucosal tissues, respectively. RESULTS: Expression of TFF2 and TFF3 in oral mucosal tissues of OSCC patien..View full abstract
This research work was supported by Khon Kaen University, the Danish Medical Research Council, and the National Health and Medical Research Council (NHMRC) and the Victorian Government's Operational Infrastructure Support Program, Australia. The authors would like to thank Professor Ebba Nexo (Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark) for research advice, Dr. Waranuch Pithipat (Department of Community Dentistry, Faculty of Dentistry Khon Kaen University, Thailand) for statistical advice, and Inger Marie Jensen (Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark) for excellent technical assistance. The authors also thank Lars Thim (Department of Protein Engineering, Novo Nordisk A/S, Malov, Denmark) for his collaboration.