Journal article

Dose-dependent recruitment of CD25( ) and CD26( ) T cells in a novel F344 rat model of asthma

Thomas Skripuletz, Andreas Schmiedl, Jutta Schade, Sammy Bedoui, Thomas Glaab, Reinhard Pabst, Stephan von Hoersten, Michael Stephan

American Journal of Physiology - Lung Cellular and Molecular Physiology | AMER PHYSIOLOGICAL SOC | Published : 2007


The ovalbumin (OVA)-induced airway inflammation in rats is a commonly used model to explore the pathobiology of asthma. However, its susceptibility varies greatly between rat strains, and presently Brown Norway (BN) rats are preferentially used. Since recruitment of T cells to the lungs depends on the CD26 (dipeptidyl peptidase IV, DPPIV) expression, Fischer 344 strain (F344) rats are a highly relevant rat strain, in particular because CD26-deficient substrains are available. To establish a F344 rat model of asthma, we challenged F344 rats using different doses of aerosolized antigen (0%, 1%, 2.5%, 5%, and 7.5% OVA) and compared these effects with intratracheal instillation of OVA (1.5 mg/0...

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