Journal article

Identification of Dendritic Cells, B Cell and T Cell Subsets in Tasmanian Devil Lymphoid Tissue; Evidence for Poor Immune Cell Infiltration into Devil Facial Tumors

Lauren J Howson, Katrina M Morris, Takumi Kobayashi, Cesar Tovar, Alexandre Kreiss, Anthony T Papenfuss, Lynn Corcoran, Katherine Belov, Gregory M Woods

Anatomical Record | WILEY | Published : 2014

Abstract

The Tasmanian devil is under threat of extinction due to the transmissible devil facial tumor disease (DFTD). This fatal tumor is an allograft that does not induce an immune response, raising questions about the activity of Tasmanian devil immune cells. T and B cell analysis has been limited by a lack of antibodies, hence the need to produce such reagents. Amino acid sequence analysis revealed that CD4, CD8, IgM, and IgG were closely related to other marsupials. Monoclonal antibodies were produced against CD4, CD8, IgM, and IgG by generating bacterial fusion proteins. These, and commercial antibodies against CD1a and CD83, identified T cells, B cells and dendritic cells by immunohistochemist..

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Grants

Awarded by ARC Linkage


Awarded by ARC Discovery


Awarded by NHMRC CDF


Awarded by NHMRC PRF


Awarded by NHMRC Program


Funding Acknowledgements

Grant sponsor: ARC Linkage; Grant number: LP0989727; Grant sponsor: Dr Eric Guiler Tasmanian Devil Research Grant; Grant sponsor: ARC Discovery; Grant number: DP110102731; Grant sponsor: NHMRC; Grant numbers: NHMRC CDF (App1003856), NHMRC PRF (App637306), and NHMRC Program (App575500).