Stability and viability of novel perennial ryegrass host-Neotyphodium endophyte associations
P Tian, T-N Le, KF Smith, JW Forster, KM Guthridge, GC Spangenberg
Crop and Pasture Science | CSIRO PUBLISHING | Published : 2013
The temperate pasture grass Lolium perenne L. is commonly found in association with the fungal endophyte Neotyphodium lolii. Viability of both seed and endophyte was evaluated by inoculation of individual genotypes from the host cultivar Bronsyn with six distinct endophyte strains. The resulting populations were subjected to either long-term storage under various temperature conditions, or accelerated ageing (AA) treatments. High temperature storage was detrimental to endophyte viability. The AA variables (relative humidity [RH] and time) and subsequent temperature and duration of storage significantly (P<0.05) affected both seed germination and endophyte viability. Significant interaction e..View full abstract
The research described here was funded by the Victorian Department of Primary Industries and New Zealand Agriseeds Ltd through the Molecular Plant Breeding and Dairy Futures Cooperative Research Centres. The authors thank several anonymous reviewers for insightful comments and suggestions during the peer-review process.