Conference Proceedings
Genetic diversity in the perennial ryegrass fungal endophyte Neotyphodium lolii
E van Zijll de Jong, NR Bannan, J Batley, KM Guthridge, GC Spangenberg, KF Smith, JW Forster, A Hopkins (ed.), ZY Wang (ed.), R Mian (ed.), M Sledge (ed.), RE Barker (ed.)
MOLECULAR BREEDING OF FORAGE AND TURF, PROCEEDINGS | SPRINGER | Published : 2004
Abstract
Expressed sequence tag (EST) data for the grass fungal endophyte species Neotyphodium lolii and N. coenophialum has been used to generate a comprehensive set of simple sequence repeat (SSR) markers for the genetic analysis of the grass-endophyte symbiosis. Initial analysis of reference isolates from the perennial ryegrass endophyte N. lolii revealed low levels of genetic variation. Twenty-two EST-SSR markers were assembled into multiplex panels for in planta detection and genetic polymorphism analysis of the endophyte. The markers were shown to be endophyte-specific and effective at low mass-ratios of endophyte to plant genomic DNA. Genetic variation has been assessed in plants obtained from..
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