Conference Proceedings
Interpretation of SNP Haplotype Complexity in White Clover (Trifolium repens L.), an Outbreeding Allotetraploid Species
Kahlil A Lawless, Michelle C Drayton, Melanie C Hand, Rebecca C Ponting, Noel OI Cogan, Timothy I Sawbridge, Kevin F Smith, German C Spangenberg, John W Forster, T Yamada (ed.), G Spangenberg (ed.)
MOLECULAR BREEDING OF FORAGE AND TURF | SPRINGER | Published : 2009
Abstract
Abstract.Single nucleotide polymorphisms (SNPs) within genic sequences provide the basis for functionally-associated genetic marker development. Gene-associated SNP discovery in white clover has been based on cloning and sequencing of PCR amplicons from parents and progeny of two-way pseudo-testcross mapping families. Target genes were selected from functional categories including phytohormone metabolism, nodulation, cell wall biosynthesis, metal binding, flavonoid biosynthesis and organic acid biosynthesis. Sequence alignments revealed haplotypic complexity that may be attributable to both paralogous gene structure and homoeologous sequence variation between sub-genomes. A high proportion o..
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