Journal article

Temporal variation of the merozoite surface protein-2 gene of Plasmodium falciparum

D Eisen, H Billman-Jacobe, VF Marshall, D Fryauff, RL Coppel

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1998


Extensive polymorphism of key parasite antigens is likely to hamper the effectiveness of subunit vaccines against Plasmodium falciparum infection. However, little is known about the extent of the antigenic repertoire of naturally circulating strains in different areas where malaria is endemic. To address this question, we conducted a study in which blood samples were collected from parasitemic individuals living within a small hamlet in Western Irian Jaya and subjected to PCR amplification using primers that would allow amplification of the gene encoding merozoite surface protein-2 (MSP2). We determined the nucleotide sequence of the amplified product and compared the deduced amino acid sequ..

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