Mannose-binding lectin deficiency influences innate and antigen-presenting functions of blood myeloid dendritic cells
Melinda M Dean, Robert L Flower, Damon P Eisen, Robyn M Minchinton, Derek NJ Hart, Slavica Vuckovic
Immunology | WILEY-BLACKWELL PUBLISHING, INC | Published : 2011
Mannose-binding lectin (MBL) is a serum lectin that plays a significant role in innate host defence. Individuals with mutations in exon 1 of the MBL2 gene have reduced MBL ligand binding and complement activation function and increased incidence of infection. We proposed that, during infection, MBL deficiency may impact on dendritic cell (DC) function. We analysed the blood myeloid DC (MDC) surface phenotype, inflammatory cytokine production and antigen-presenting capacity in MBL-deficient (MBL-D) individuals and MBL-sufficient (MBL-S) individuals using whole blood culture supplemented with zymosan (Zy) or MBL-opsonized zymosan (MBL-Zy) as a model of infection. Zy-stimulated MDCs from MBL-D ..View full abstract
We would like to acknowledge the Australian Red Cross Blood Service (the Blood Service) for the provision of the MBL used in this study, and the Australian Government, which fully funds the Blood Service for the provision of blood products and services to the Australian community.