Characterization of innate responses to influenza virus infection in a novel lung type I epithelial cell model
Carrie M Rosenberger, Rebecca L Podyminogin, Peter S Askovich, Garnet Navarro, Shari M Kaiser, Catherine J Sanders, Jennifer L McClaren, Vincent C Tam, Pradyot Dash, Jhoanna G Noonan, Bart G Jones, Sherri L Surman, Jacques J Peschon, Alan H Diercks, Julia L Hurwitz, Peter C Doherty, Paul G Thomas, Alan Aderem
Journal of General Virology | SOC GENERAL MICROBIOLOGY | Published : 2014
Type I alveolar epithelial cells are a replicative niche for influenza in vivo, yet their response to infection is not fully understood. To better characterize their cellular responses, we have created an immortalized murine lung epithelial type I cell line (LET1). These cells support spreading influenza virus infection in the absence of exogenous protease and thus permit simultaneous analysis of viral replication dynamics and host cell responses. LET1 cells can be productively infected with human, swine and mouse-adapted strains of influenza virus and exhibit expression of an antiviral transcriptional programme and robust cytokine secretion. We characterized influenza virus replication dyna..View full abstract
Awarded by NIH
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
We thank Nick Lejarcegui and Hieu Nguyen at the Seattle BioMed Flow Cytometry facility for technical assistance. The authors have no conflicting financial interests. This work was funded by NIH grant HHSN272200800058C.