Purification and biological characterization of soluble, recombinant mouse IFN beta expressed in insect cells
Sebastian A Stifter, Jodee A Gould, Niamh E Mangan, Hugh H Reid, Jamie Rossjohn, Paul J Hertzog, Nicole A de Weerd
Protein Expression and Purification | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2014
Interferon β (IFNβ) is a member of the type I interferon family of cytokines widely recognised for their anti-viral, anti-proliferative and immunomodulatory properties. Recombinant, biologically active forms of this cytokine are used clinically for the treatment of multiple sclerosis and in laboratories to study the role of this cytokine in health and disease. Established methods for expression of IFNβ utilise either bacterial systems from which the insoluble recombinant proteins must be refolded, or mammalian expression systems in which large volumes of cell culture are required for recovery of acceptable yields. Utilising the baculovirus expression system and Trichoplusia ni (Cabbage Loope..View full abstract
The authors thank Dr. Ashley Mansell of MIMR for the gift of RAW 264.7 cell line and Dr. Rebecca Smith of MIMR for manuscript and submission assistance. This work is supported by funding from the National Health and Medical Research Council (NHMRC) and the Victorian Government's Operational Infrastructure Support Program. NEM is supported by funding from the NHMRC and Australian Research Council (ARC). HHR is supported by funding from NHMRC. JR is supported by an NHMRC Australia Fellowship. PJH is supported by an NHMRC Senior Principal Research Fellowship.