Polarized Secretion of PEDF from Human Embryonic Stem Cell-Derived RPE Promotes Retinal Progenitor Cell Survival
Danhong Zhu, Xuemei Deng, Christine Spee, Shozo Sonoda, Chih-Lin Hsieh, Ernesto Barron, Martin Pera, David R Hinton
Investigative Ophthalmology & Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2011
PURPOSE: Human embryonic stem cell-derived RPE (hES-RPE) transplantation is a promising therapy for atrophic age-related macular degeneration (AMD); however, future therapeutic approaches may consider co-transplantation of hES-RPE with retinal progenitor cells (RPCs) as a replacement source for lost photoreceptors. The purpose of this study was to determine the effect of polarization of hES-RPE monolayers on their ability to promote survival of RPCs. METHODS: The hES-3 cell line was used for derivation of RPE. Polarization of hES-RPE was achieved by prolonged growth on permeable inserts. RPCs were isolated from 16- to 18-week-gestation human fetal eyes. ELISA was performed to measure pigment..View full abstract
Awarded by California Institute for Regenerative Medicine
Supported in part by Grants RS 1-00222-1 and DR1-01444 from the California Institute for Regenerative Medicine and by the Arnold and Mabel Beckman Foundation.