Journal article

Silencing of Odorant Receptor Genes by G Protein beta gamma Signaling Ensures the Expression of One Odorant Receptor per Olfactory Sensory Neuron

Todd Ferreira, Sarah R Wilson, Yoon Gi Choi, Davide Risso, Sandrine Dudoit, Terence P Speed, John Ngai

Neuron | CELL PRESS | Published : 2014

Abstract

Olfactory sensory neurons express just one out of a possible ∼ 1,000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here, we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein βγ subunits is both necessary and sufficient to suppress the expressi..

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University of Melbourne Researchers

Grants

Awarded by National Human Genome Research Institute


Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES


Awarded by NATIONAL HUMAN GENOME RESEARCH INSTITUTE


Awarded by NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS


Funding Acknowledgements

This work was supported by grants to J.N. from the National Institute on Deafness and Other Communication Disorders. T. F. was also supported by a training grant from the National Human Genome Research Institute (T32 HG00047). S. R. W. was the recipient of a Graduate Research Fellowship from the National Science Foundation. We thank the members of the Ngai lab, past and present, for their invaluable advice and suggestions over the course of this study.