An Antibody-Based Leukocyte-Capture Microarray for the Diagnosis of Systemic Lupus Erythematosus
Ming-Wei Lin, Joshua WK Ho, Leonard C Harrison, Cristobal G dos Remedios, Stephen Adelstein
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013
The diagnosis of Systemic Lupus Erythematosus (SLE) is challenging due to its heterogeneous clinical presentation and the lack of robust biomarkers to distinguish it from other autoimmune diseases. Further, currently used laboratory tests do not readily distinguish active and inactive disease. Several groups have attempted to apply emerging high throughput profiling technologies to diagnose and monitor SLE. Despite showing promise, many are expensive and technically challenging for routine clinical use. The goal of this work is to develop a better diagnostic and monitoring tool for SLE. We report a highly customisable antibody microarray that consists of a duplicate arrangement of 82 antibod..View full abstract
Studies were supported by the NWG Macintosh Memorial Fund of the Department of Anatomy & Histology, University of Sydney and the Special Purposes Trust Fund and generous donors to the Department of Clinical Immunology, Royal Prince Alfred Hospital. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.