Definition of High-Risk Type 1 Diabetes HLA-DR and HLA-DQ Types Using Only Three Single Nucleotide Polymorphisms
Nguyen Cao, Michael D Varney, Leonard C Harrison, Grant Morahan
DIABETES | AMER DIABETES ASSOC | Published : 2013
Evaluating risk of developing type 1 diabetes (T1D) depends on determining an individual's HLA type, especially of the HLA DRB1 and DQB1 alleles. Individuals positive for HLA-DRB1*03 (DR3) or HLA-DRB1*04 (DR4) with DQB1*03:02 (DQ8) have the highest risk of developing T1D. Currently, HLA typing methods are relatively expensive and time consuming. We sought to determine the minimum number of single nucleotide polymorphisms (SNPs) that could rapidly define the HLA-DR types relevant to T1D, namely, DR3/4, DR3/3, DR4/4, DR3/X, DR4/X, and DRX/X (where X is neither DR3 nor DR4), and could distinguish the highest-risk DR4 type (DR4-DQ8) as well as the non-T1D-associated DR4-DQB1*03:01 type. We analy..View full abstract
Awarded by National Health and Medical Research Council of Australia
Awarded by National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
This work was supported by program grants 53000400 and 37612600 from the National Health and Medical Research Council of Australia and by The Diabetes Research Foundation of Western Australia. C.N. was supported by grant 1DP3DK085678-01 from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) and is supported by The Diabetes Research Foundation of Western Australia. This research utilizes resources provided by the Type 1 Diabetes Genetics Consortium, a collaborative clinical study sponsored by NIDDK, National Institute of Allergy and Infectious Diseases, National Human Genome Research Institute, National Institute of Child Health and Human Development, and Juvenile Diabetes Research Foundation International and supported by U01 DK062418.