Reconstruction of the nigrostriatal dopamine pathway in the adult mouse brain
Lachlan H Thompson, Shane Grealish, Deniz Kirik, Anders Bjorklund
European Journal of Neuroscience | WILEY | Published : 2009
Transplants of fetal dopamine neurons can be used to restore dopamine neurotransmission in animal models of Parkinson's disease, as well as in patients with advanced Parkinson's disease. In these studies the cells are placed in the striatum rather than in the substantia nigra where they normally reside, which may limit their ability to achieve full restoration of motor function. Using a microtransplantation approach, which allows precise placement of small cell deposits directly into the host substantia nigra, and fetal donor cells that express green fluorescent protein under the control of the tyrosine hydroxylase promoter, we show that dopamine neuroblasts implanted into the substantia nig..View full abstract
Awarded by Swedish Research Council
Awarded by European Union
This work was supported by grants from the Swedish Research Council (04X-3874) and the Knut and Alice Wallenberg Foundation. L.H.T. was supported by grants from the National Parkinson Foundation (U.S.) Individual-Investigator grants program and the NeuroNE Network of Excellence program of the European Union (LSHM-CT-2004-512039). We thank Drs Hideyuki Okano and Kazuto Kobayashi for providing the TH-GFP mice. We are extremely grateful to Bjorn Anzelius, Stephan Hermening, Ulla Jarl, Anneli Josefsson and Elsy Ling for technical assistance, and Bengt Mattsson for preparation of the illustrative material.