Journal article

Lentivirus-mediated gene transfer to the rat, ovine and human cornea

DGA Parker, C Kaufmann, HM Brereton, DS Anson, L Francis-Staite, CF Jessup, K Marshall, C Tan, R Koldej, DJ Coster, KA Williams

Gene Therapy | NATURE PUBLISHING GROUP | Published : 2007

Abstract

Gene therapy of the cornea shows promise for modulating corneal transplant rejection but the most appropriate vector for gene transfer has yet to be determined. We investigated a lentiviral vector (LV) for its ability to transduce corneal endothelium. A lentivector expressing enhanced yellow fluorescent protein (eYFP) under the control of the Simian virus type 40 early promoter (LV-SV40-eYFP) transduced 80-90% of rat, ovine and human corneal endothelial cells as detected by fluorescence microscopy. The kinetics of gene expression varied among species, with ovine corneal endothelium showing a relative delay in detectable reporter gene expression compared with the rat or human corneal endothel..

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