Journal article

INTERLEUKIN-4 SUPPRESSES GRANULOCYTE COLONY-STIMULATING FACTOR AND GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR LEVELS IN STIMULATED HUMAN MONOCYTES

JA HAMILTON, GA WHITTY, AKM ROYSTON, J CEBON, JE LAYTON

IMMUNOLOGY | BLACKWELL SCIENCE LTD | Published : 1992

Abstract

Granulocyte colony-stimulating factor (G-CSF) was quantitated in the supernatants of lipopolysaccharide (LPS)-treated human monocytes by ELISA. Unlike previous reports, the lymphokines, interleukin-4 (IL-4) and interferon-gamma (IFN-gamma), were unable to induce the synthesis of G-CSF. Both IL-4 (> or = 10 pM) and the glucocorticoid, dexamethasone (10(-7) M), inhibited G-CSF production in the LPS-treated monocytes; in contrast, IFN-gamma had a weak potentiating effect on the LPS action. Changes in antigen expression were manifested at the level of messenger RNA (mRNA). Granulocyte-macrophage (GM)-CSF in the LPS-treated monocyte supernatants was also quantitated by ELISA but its levels were s..

View full abstract